Small rna sorting matchmaking for argonautes

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For AtAGO2, as with AtAGO1, there are no crystal contacts that impede nucleotide binding. Viroids rely entirely on host machinery for replication. Competing interests: The authors have declared that no competing interests exist. Therefore, it is crucial that these very similar species are appropriately sorted among closely related partners.

However, mammalian cells are still capable of generating miRNAs in the absence of the microprocessor. RNA processing by DCL2 results in small 22 nt siRNAs. We have begun to piece together the custodes that determine small RNA fates and, in some instances, these properties can even predict with reasonable accuracy which small RNAs will efficiently join a particular effector complex. Sampson et al 2013 have revealed that in pathogenic Francisella bacteria, the Cas9 protein guided by small RNAs represses the mRNA of a lipoprotein. MicroRNAome of sincere macrophages in hypersplenism due to portal hypertension in hepatitis B virus-related cirrhosis. Identifying functional miRNA—mRNA regulatory modules with correspondence latent dirichlet allocation. Challenges and Future Aspects Early application of the first generation SRGE involves expression of functional viral products, which raises concerns to the human health and the environment.

In this review we summarized the efforts generating viral resistance with SRGE in different crops, analyzed the evolution of the technology, its efficacy in different crops for different viruses and its application status in different crops. Inactivation of daf-16, however, completely suppressed the extended lifespan of alg-2 mutants, demonstrating a clear link between alg-2 and daf-16 in lifespan extension. In the first two generations of small RNA technology, the mature small RNAs that function in viral immunity are not predefined. In the generation of both groups, a precursor molecule is processed into a short double-stranded RNA dsRNA duplex by a member of the Dicer family of endonucleases.

Login using - Together, these studies suggest that virally-encoded miRNAs are an important aspect in the maintenance of KSHV latency.

Small RNAs directly or indirectly impact nearly every biological process in eukaryotic cells. To perform their myriad roles, not only must precise small RNA species be generated, but they must also be loaded into specific effector complexes called RNA-induced silencing complexes RISCs. Argonaute proteins form the core of RISCs and different members of this large family have specific expression patterns, protein binding partners and biochemical capabilities. In this Review, we explore the mechanisms that pair specific small RNA strands with their partner proteins, with an eye towards the substantial progress that has been recently made in understanding the sorting of the major small RNA classes - microRNAs miRNAs and small interfering RNAs siRNAs - in plants and animals. Small RNAs directly or indirectly impact nearly every biological process in eukaryotic cells. To perform their myriad roles, not only must precise small RNA species be generated, but they must also be loaded into specific effector complexes called RNA-induced silencing complexes RISCs. Argonaute proteins form the core of RISCs and different members of this large family have specific expression patterns, protein binding partners and biochemical capabilities. In this Review, we explore the mechanisms that pair specific small RNA strands with their partner proteins, with an eye towards the substantial progress that has been recently made in understanding the sorting of the major small RNA classes — microRNAs miRNAs and small interfering RNAs siRNAs — in plants and animals. The discovery of RNA interference RNAi in the late 1990s sparked a renaissance in our understanding of RNAs as regulatory molecules. A growing number of small RNA classes has since emerged from studies of eukaryotic organisms, and these RNAs can be approximately divided into two groups: small RNAs that engage RNAi-related machinery and those that do not. As yet, we know very little about many newly discovered groups of small RNAs, but our understanding of the biogenesis and biological functions of RNAi-related small RNA classes is growing rapidly. Small RNAs that engage RNAi-related pathways share several characteristic features. The precise combination of a small RNA with a particular Argonaute protein determines its biological function. Therefore, it is crucial that these very similar species are appropriately sorted among closely related partners. Only then can the target specificity conferred on Argonaute proteins by their small RNA guides enable their myriad important roles, which include the regulation of gene expression, modification of chromosome structure and protection from mobile elements. Conceptually, all small RNA-mediated regulatory events can be considered as the culmination of several consecutive steps: small RNA biogenesis, strand selection in which dsRNA is the precursor , loading into Argonaute, target recognition and effector function. The biogenesis of most small RNA classes, including microRNAs miRNAs and many small interfering RNAs siRNAs , requires the action of RNase III family proteins reviewed in REFS —. Some small RNA classes, including Piwi-interacting RNAs piRNAs and secondary siRNAs in worms, however, are not derived from dsRNA precursors and are produced through alternative biogenesis mechanisms independently of RNase III enzymes -. Following their production, small RNAs are sorted to confer association with specific Argonaute family proteins, which function as the core of the rna-induced silencing complex RISC. Argonaute proteins can be classified into three subgroups according to their sequence relationships: the AGO subfamily, the Piwi subfamily and the worm-specific WAGO clade -. Piwi subfamily proteins load small RNAs derived from single-stranded precursors piRNAs and AGO clade proteins usually associate with small RNA duplexes processed by RNase III endonucleases miRNAs and siRNAs; reviewed in REFS ,. Small RNAs that occupy WAGO clade proteins are usually direct products of RNA synthesis ,,. Mature RISC consists of a single-stranded small RNA bound to an Argonaute protein. As some small RNAs are generated as duplexes, only one strand the guide strand is retained and the other passenger strand is discarded during RISC assembly -. AGO clade proteins are generally loaded with small RNA duplexes before RISC maturation. Thus, it is of key importance to assemble RISC in a manner that ensures that the appropriate guide strand is selectively stabilized, as loading of the passenger strand would obviously misdirect RISC towards inappropriate targets. Small RNAs guide mature RISC through complementary base pairing to its targets, with the most common outcome being target repression reviewed in REFS -. The knowledge of the mechanisms that guide a particular small RNA strand into a specific Argonaute family member is crucial. It impacts our ability to predict the biological function of a small RNA and to effectively use small RNAs as experimental tools or therapeutics. This Review focuses on our understanding of small RNA sorting in plants and animals. We consider biogenesis as a starting point as this affects the nature of small RNAs and, in some cases, the complexes which the small RNAs join. Next, we discuss the small RNA-intrinsic determinants of sorting, followed by RISC loading and maturation. Finally, we briefly cover the implications of sorting for Argonaute function. We do not extensively discuss the effector mechanisms of mature RISC, but instead refer the reader to several excellent recent reviews on this topic -. Small RNA biogenesis In effect, the first step of small RNA sorting is biogenesis, as this determines the small RNAs that are available for RISC loading. Moreover, the precise enzymes that liberate small RNAs from their precursor transcripts or generate them de novo seem to impact the choice of their ultimate Argonaute partner. Therefore, it is important to begin with an introduction to the varied mechanisms that can produce small RNAs. Small RNA duplexes from partial or perfect dsRNA precursors are generated by RNase III family enzymes through sequential endonucleolytic cleavage events. These enzymes often partner with dsRNA binding domain dsRBD proteins, which serve to increase substrate specificity and affinity, leading to increased activity. Animal miRNA processing miRNAs are ubiquitous in animal genomes and are often transcribed as separate coding units, many of which consist of polycistronic clusters containing multiple miRNAs. Some miRNAs are also present in introns and presumably arise from further processing of the excised introns of proteincoding genes. Most miRNAs are transcribed by DNA-dependent RNA polymerase II RNAPII to generate a primary miRNA pri-miRNA containing a region of imperfect dsRNA, known as the stem—loop structure, that harbours the future mature miRNA ,. Primary miRNA transcripts seem largely like the transcripts of protein-coding genes. The production of conventional miRNAs from these precursors proceeds through two site-specific cleavage events. This complex recognizes the duplex character of the pri-miRNA, although the precise RNA—protein interactions that select pri-miRNAs as Microprocessor substrates and how the cleavage site is determined by these interactions are matters of ongoing work. The pri-miRNA is cleaved by Drosha to liberate a ~60—70-nt precursor miRNA pre-miRNA from the primary transcript. Additional factors, including the nuclear export receptor exportin 1 XPO1 , the cap-binding complex CBC and the Arabidopsis thaliana SERRATE homologue, ARSENITE-RESISTANCE PROTEIN 2 ARS2 , were recently suggested to play a part in the transition from pri- to pre-miRNA -. For this purpose, the sole mammalian Dicer partners with the dsRBD protein TAR RNA-binding protein 2 TARBP2, also known as TRBP ,, whereas the Drosophila melanogaster miRNA-generating Dicer 1 DCR1 similarly interacts with a specific isoform of its dsRBD protein partner loquacious LOQS-PB -. Several unconventional miRNAs that are defined by their use of alternative maturation strategies have now been noted. For example, mirtrons have been found in flies and mammals -. Mirtrons bypass the Drosha processing step and instead use the splicing machinery to generate pre-miRNAs. Mirtrons are very short introns and are excised, debranched and refolded into short stem—loop structures that mimic pre-miRNAs and are processed into mature miRNAs by Dicer. Plant pri-miRNAs typically display greater diversity in the size and structure of their stem—loops compared with their animal counterparts. As in animals, Dicer is assisted by a dsRBD protein, in this case, HYPONASTIC LEAVES 1 HYL1 -. HYL1 and the zinc finger protein SERRATE promote accurate miRNA processing ,-. Plant microRNA-processing mechanisms Plant microRNAs miRNAs are generally produced by sequential rounds of Dicing. This is necessitated by the lack of a Drosha orthologue. The extensive nature of the hairpins that lead to many plant miRNAs also permits phased production of multiple small RNA duplexes through sequential Dicing events, conceptually the plant version of long hairpin endogenous small interfering RNA siRNA precursors or miRNA polycistrons in animals. For example, miR319 and miR159 both with conserved long precursors are produced by an unusual loop-to-stem mechanism. Following the first cleavage of the loop by DCL1, consecutive cuts by DCL1 are necessary to release the mature miRNA duplex. CBC, cap-binding complex; DDL, DAWDLE; HEN1, HUA ENHANCER 1; HYL1, HYPONASTIC LEAVES 1; SE, SERRATE. Maturation of plant miRNA duplexes often proceeds through several rounds of sequential Dicing from the base of a long stem—loop. Processed miRNA duplexes are modified by the methyltransferase HUA ENHANCER 1 HEN1 -. In contrast to its D. This adaptation may be necessitated by the fact that plant miRNAs pair extensively with target mRNAs and cleave them, a process which in animals provides a trigger for small RNA destruction. However, the exact form of the exported cargo and the subcellular localization of plant RISC loading and maturation remain subjects of current debate. In this regard, a recent study proposed a model in which RISC is assembled in the nucleus and only mature AGO1—RISC containing a single-stranded miR can be exported to the cytoplasm. The earliest identified examples were derived from viral replication intermediates or complex interactions between transgene copies. By considering the commonalities between these origins, dsRNAs were indicated as the source of small RNAs. It is now clear that plants and animals produce a wide range of siRNAs. These vary in their biogenesis mechanisms, but can be approximately divided into two classes, depending on whether they require RNA-dependent RNA polymerases RdRPs for their production. Here, the experimental introduction of long dsRNAs results in the production of exo-siRNAs that are ~21 nt in size. Dicer 2 also interacts with another dsRBD protein R2D2, but only LOQS-PD enhances siRNA production ,. Recent studies indicate a role of R2D2 in loading siRNA duplexes into RISC discussed below , suggesting that these two dsRBD proteins may have distinct and sequential functions ,. Production of small interfering RNAs In flies, siRNAs also originate from numerous endogenous loci and were termed endogenous siRNAs endo-siRNAs -. These can originate from RNA transcripts with extensive hairpin structures, from convergent transcription units similar to plant nat-siRNAs, see below or from the annealing of sense and antisense RNAs from unlinked loci. One example of the latter type of siRNAs are endo-siRNAs that target transposons, which seem to arise at least in part from the hybridization of transposon mRNAs with piRNA cluster transcripts. Another possible source of dsRNA hybrids is the interaction of sense and antisense transcripts across individual transposon copies, and it has even been suggested that RdRPs may operate in animals to form dsRNAs. As with exo-siRNAs, the biogenesis of endo-siRNAs depends on Dicer 2 assisted by LOQS-PD ,,-. A similar situation has been described in mammals; however, the range of cell types in which dsRNAs are produced and converted into siRNAs seems to be limited. Thus far, endo-siRNAs have been detected in abundance only in mouse oocytes and embryonic stem ES cells -. The dsRNA triggers that give rise to murine endo-siRNAs are predicted to arise from trans interactions between gene and pseudogene transcripts, from overlapping transcription units and from transcripts that can form long hairpins. As in flies, endo-siRNA biogenesis is dependent on Dicer and, presumably, its dsRBD partners. RdRP-dependent siRNAs In contrast to mammals and flies, worms and plants produce numerous endo-siRNAs using biogenesis mechanisms that depend on the action of RdRPs. Plant RdRPs copy single-stranded precursors into long dsRNAs that are cleaved by Dicer, whereas worm RdRPs can directly synthesize siRNAs without Dicer processing. Primary siRNAs in Caenorhabditis elegans are produced conventionally, from long dsRNA triggers through the action of DCR-1 REFS ,,. The siRNAs associate with the Argonaute family protein, RDE-1 and guide it to target transcripts. The RDE-1—target interaction recruits an RdRP, an outcome that is independent of RDE-1 catalytic activity. The RdRP uses the target as template for the synthesis of secondary siRNAs of 22— 24 nt. The production of most plant siRNAs requires the action of RdRPs to convert ssRNA precursors to dsRNA triggers. Three major subclasses of endogenous siRNAs can be distinguished in plants: trans-acting siRNAs ta-siRNAs , natural antisense transcript-derived siRNAs nat-siRNAs and heterochromatic siRNAs hc-siRNAs. Each of these small RNA subclasses is produced by a specific Dicer family member and preferentially loaded into a distinct AGO complex. The biogenesis of ta-siRNAs requires the interplay of canonical components of miRNA and siRNA processing -. The process begins with miRNA-mediated cleavage of the TAS1 or TAS3 non-coding RNAs by miR390—AGO7 or miR173—AGO1, respectively. This triggers the recruitment of SUPPRESSOR OF GENE SILENCING 3 SGS3 and RNA-DEPENDENT RNA POlYMERASE 6 RDR6 , which synthesizes dsRNA using the cleavage site as the entry point. The resulting dsRNA is processed by DCl4 and its dsRBD protein partner DRB4 into a phased series of 21-nt siRNA duplexes, which begins at the site of initial cleavage. The subcellular localization of biogenesis factors and RNA intermediates, along with the recruitment of SDE5 a putative export factor homologue , suggests that ta-siRNA biogenesis might involve specific nuclear—cytoplasmic shuttling ,. Plant genomes often possess convergent transcription units that can give rise to dsRNA. Under certain conditions, often resulting from biotic and abiotic stress, bidirectional transcription is induced and the resulting dsRNA is processed into nat-siRNAs -. Production of nat-siRNAs requires DCL2 which produces 24-nt siRNAs or DCL1 resulting in 22-nt siRNAs , depending on the genomic origin of the overlapping transcripts. Other essential biogenesis factors include RDR6, SGS3, HYL1, HEN1 and RNAPIV ,. A highly abundant class of plant endo-siRNAs — hc-siRNAs — arises from repeats and transposable elements -. Processed siRNA duplexes are methylated by HEN1 and primarily loaded into AGO4. Structural determinants of Argonaute proteins for small RNA sorting Argonaute AGO proteins provide numerous possibilities for RNA—protein interactions that might underlie the proposed determinants of small RNA strand sorting. The interaction between AGOs and small RNAs occurs through several contact points in three characteristic domains of the protein: the PAZ, Mid and PIWI domains a and b; part b shows a stereo view of the crystal structure of Thermus thermophilus AGO bound to a guide DNA—target RNA duplex. Interestingly, this structure is well conserved in all four human AGO proteins as well as in Drosophila melanogaster AGO1 or the worm miRNA acceptors ALG-1 and ALG-2. By contrast, AGO proteins that function in other small RNA pathways, such as D. The PIWI domain, which shows similarity to RNase H folds, harbours the residues required for catalytic activity in AGO protein usually Asp—Asp—His. Thus, cleavage-competent AGO proteins carry out endonucleolytic cleavage of target transcripts through their PIWI domain ,-. Panel b is reproduced from Ref. © 2008 Macmillan Publishers Ltd. In part, sorting may be driven by specific protein—protein interactions between biogenesis and effector components. For example, in animals, Dicing and Argonaute loading have been proposed to occur as concerted processes ,. This provides an opportunity for determining the fate of specific precursors to join certain effector complexes if a particular Dicer preferentially binds one Argonaute family member. However, Dicer and Argonaute cannot be the full story. Instead, it is clear that more complex-loading and strand-recognition pathways also influence the sorting of small RNAs. To exert its regulatory functions, mature RISC must be programmed with a single-stranded RNA. Thus, for small RNAs that are initially produced as duplexes, one strand must be chosen and the other discarded — a process called RISC loading. Strand selection must not be random. Even from the first mechanistic studies, it was clear that strand choice was partly encoded in the intrinsic structure of the small RNA duplex, and a major determinant resides in its thermodynamic properties ,. There are also additional favourable sequence characteristics, such as a bias for a U at position 1 see for further details ,,-. Recently, our understanding of small RNA-sorting determinants has expanded substantially, and Argonaute and RNA structural studies have begun to provide a mechanistic basis for observations from in vitro and in vivo analyses ,,,-. Small RNA sorting in animals In mammals, a single Dicer assorts siRNAs and miRNAs among four Argonaute subfamily proteins, apparently without much discrimination. Generally, AGO1 is occupied by miRNAs, whereas AGO2 associates with siRNAs. This parallels the processing of miRNAs by Dicer 1 and siRNAs by Dicer 2. However, there are exceptions to the rule. For example, there are Dicer 1-derived small RNAs that preferentially load AGO2, implying the existence of a post-processing sorting mechanism ,. Other features that affect sorting include the terminal nucleotides and thermodynamic properties of the duplex ends. Small RNA sorting and RNA-induced silencing complex assembly in flies The numerous inputs into the sorting decisions of small RNAs have posed a challenge to predicting their fates in D. However, recent studies have suggested the application of hierarchical rules to predict differential AGO loading ,,. At the top level is duplex structure, specifically its degree of base pairing. Small RNA strands with unpaired central regions ~nucleotides 9—10 tend to be directed into AGO1 and disfavoured for AGO2 loading. For perfect duplexes, thermodynamic asymmetry dominates strand choice, which is precisely as was originally proposed ,,. It should be noted that sorting is a strand-centric process. Once a duplex is made, it seems that one strand is assessed and its fate determined. It was recently noted in D. Interestingly, in vitro, these small RNAs are sorted into AGO1. In vivo, however, these AGO1-loaded endo-siRNAs silence targets with high sequence complementarity. Although worm sorting rules have not been probed in detail, miRNAs show a tendency towards central mismatches and are sorted into ALG-1 or ALG-2, whereas siRNAs from perfect duplexes preferentially load RDE-1 REFS ,. This raises the possibility that mammals lack a strict system for small RNA sorting, at least among their AGO subfamily members. Sorting of small RNAs in plants A. As in animals, plant AGO proteins tend to show preferences for distinct small RNA classes, which are produced through somewhat compartmentalized biogenesis pathways. For example, AGO1 is manly occupied by miRNAs that arise through processing by DCL1. AGO4 prefers hc-RNAs that are processed by DCL3. AGO2 is the principal recipient for ta-siRNAs. An additional complexity is that different Dicers produce small RNAs of distinct sizes. Plant DCL1 and DCL4 produce 21-nt RNAs, DCL2 22-nt RNAs and DCL3 24-nt RNAs. Different Dicer proteins have also been proposed to reside in different subcellular compartments. Thus, a wide range of properties might be exploited to establish specificity in plant small RNA sorting. Surprisingly, although the terminal nucleotide of the siRNA had a minor effect on sorting in flies and mammals, it strongly impacts sorting in plants. AGO1 showed a strong bias towards a terminal U. Simply changing the terminal nucleotides could redirect small RNAs into different complexes in a predictable manner, strongly supporting the dominance of this sorting signal. There were exceptions to the simple rule proposed above. MiR390, which begins with an A, would be predicted to load AGO2 but, instead, exclusively occupied AGO7 REF. Moreover, miR390 could not be redirected by altering its terminal base. Thus, although base recognition contributes strongly to sorting, other characteristics of small RNAs must also be taken into account. These could include duplex properties, such as thermodynamic asymmetry or degree of base pairing, although this hypothesis has yet to be examined. Overall, the data support a model in which plant small RNAs dissociate following Dicer cleavage and are subject to a sorting process, which surveys their terminal base. Other considerations, their size and the Dicer that produced them may contribute to specificity in a manner that varies with the small RNA species, but which becomes the dominant determinant of sorting in a few instances. Sorting of other small RNA classes To date, we know far more about the loading determinants of miRNAs and siRNAs than of any other small RNA class. Even within these well-studied groups, there are exceptions to the rules outlined above. For example, several reports now support the idea that pre-miRNA hairpins can be successfully loaded into RISC ,-. Mirtrons bypass the Drosha step but are presumably loaded using the normal miRNA strand determinants following Dicer cleavage. It had been reported in the literature that precursor-microRNA pre-miRNA hairpins are sometimes directly loaded into RNA-induced silencing complex RISC instead of being funnelled into the canonical Dicer-dependent biogenesis pathway ,,. Recently, it was shown that this strategy is actually used as a biogenesis mechanism by a conserved vertebrate miRNA, miR-451 REFS -. Like other endogenous miRNAs, mir-451 is synthesized by RNA polymerase II RNAPII as a polycistronic transcript together with mir-144 see the figure above. This primary miRNA pri-miRNA is initially processed by the Microprocessor Drosha—Pasha complex through the canonical biogenesis pathway. However, following export to the cytoplasm, the two pre-miRNAs adopt distinct fates. Although pre-mir-144 continues along the canonical miRNA path and is processed by Dicer, pre-mir-451 is not a Dicer substrate, perhaps because its 17-nucleotide nt -duplexed region is too short. Instead, the pre-mir-451 hairpin is directly loaded into Argonaute 2 AGO2. There, the duplexed portion of the hairpin is cleaved by the Argonaute RNase H-like motif and the cleaved product is resected by an unknown activity to form mature miR-451. Although it is unclear whether pre-mir-451 is actively sorted into AGO2, only those species which occupy this catalytically competent AGO family member can mature. As a second example, the pre-miRNA equivalents for mirtrons are formed by the splicing machinery rather than by Drosha. Their biogenesis is outlined in. Several small RNA classes are formed without a double-stranded precursor. Even though this should pose a simpler sorting problem, with no need to discriminate guide versus passenger strands, we know little about how these species are selectively loaded into specific Argonautes. Among good examples are the secondary siRNAs in worms, which are generated as direct RdRP products, presumably without the need for further processing ,. These are specifically loaded into WAGO clade Argonautes through a still mysterious mechanism. The loading of these small RNAs into Piwi subfamily proteins and the requirements of associated partner proteins for proper Piwi—RISC assembly are unknown. Whether the striking bias for a terminal U seen in many piRNAs reflects upstream processing activities or is a consequence of the nucleotide-binding preferences of these Piwi proteins as is seen in plants remains unclear. The RISC-loading machinery Small RNA duplexes cannot be efficiently incorporated into AGO proteins without assistance from additional proteins ,. These factors are also known as the RISC-loading machinery or pre-RISC and their precise nature differs for distinct AGO proteins. RISC loading is an active process that requires ATP ,-, probably owing to the necessity to drive conformational changes so that AGO proteins accept small RNA duplexes. This concept, which was originally suggested based on structural analyses of AGO proteins, has gained recent support from studies that characterized interactions between Argonautes and the heat shock cognate 70 HSC70 —heat shock protein 90 HSP90 chaperone complex -. In flies, the loading machinery for AGO2—RISC also involves Dicer 2 and its dsRBD partner R2D2 REFS ,,,,,. In fact, these factors have been proposed to be the biochemical sensors for thermodynamic asymmetry. In this regard, R2D2 has been shown to bind the more stable end of the dsRNA duplex, whereas Dicer 2 is positioned at the less-stable end of the duplex, providing a mechanism for orientated AGO2 loading. Although a minimal pre-RISC could be constituted with only Dicer 2, R2D2 and AGO2 REF. Roles for Dicers have also been suggested for AGO1 loading. One report suggests that AGO1—Dicer 1 complexes correspond to the AGO1—RISC-loading complex , whereas a second report indicated that Dicer 1 was dispensable for AGO1—RISC assembly. Although little is known about the loading machinery in plants, a recent study proposed that the thermodynamic properties of duplex ends instead of terminal nucleotides are the dominant determinant for strand selection of some DCL1-processed miRNAs and that HYL1, like fly R2D2, functioned as a component of the asymmetry sensor. RISC maturation For RISC to exert its function, pre-RISC needs to mature. In flies and mammals, distinct AGO proteins seem to achieve this by different mechanisms, which depend on the nature of the AGO protein and the degree of base pairing in the loaded duplex. The cleaved strand dissociates from RISC and, in flies, is degraded by a multimeric endonuclease complex consisting of Translin and Trax , termed C3PO component 3 promoter of RISC. Maturation of miRNA RISC is less well understood , bottom. Human AGO1, AGO3 and AGO4 all lack slicer activity and fly AGO1 is a poor slicer. The impact of sorting on target regulation The ultimate result of accurate strand selection and sorting is that an active RISC is formed, which is imbued with the ability to regulate a target gene or process. Argonaute family members differ in their biochemical properties, subcellular localization and expression patterns, and matching the right small RNA with the correct partner is key to proper biological function. Although AGO proteins evolved as ribonucleases, animal miRNAs affect their targets without the need for this activity. The prevalence of cleavage-independent repression modes is also reflected in the diversity of the Argonaute family. In mammals, three of the four AGO proteins have lost catalytic potential, and AGO1, the D. However, this is assumed to be the principal regulatory mode for endo-siRNAs and for piRNA-mediated repression of transposons. Here again, the choice of a particular AGO partner is crucial. Piwi family members all retain catalytic competence and D. Individual Argonaute AGO proteins differ in their expression patterns, subcellular localization and enzymatic properties. Thus, distinct AGOs can function through many different effector modes that may involve slicing of target transcripts, cleavage-independent regulation and chromatin modification reviewed in REFS -. Another layer of complexity is added by the degree of sequence complementarity between the AGO-bound small RNA and target transcripts, which determines the mechanism of regulation. Owing to limited sequence complementarity between the small RNA seed region and the mRNA, such interactions usually do not result in direct cleavage of the targeted transcript. Instead, AGO1 and its partner protein GW182 are likely to disrupt crucial interactions between the polyA tail and the cap of the transcript, leading to a reduction in translational initiation and an induction of mRNA decay. In mammals, it was recently shown that reduced protein output is predominantly owing to destabilization of the target transcript. AGO2 primed with a small RNA sharing extensive complementarity with its target typically directs endonucleolytic cleavage of the mRNAs through AGO2 slicer activity. The 2- O-methyl modification of AGO2-bound small RNAs prevents their degradation when targeting perfectly complementary transcripts ,,. However, other modes are possible: AGO2 can also regulate targets with limited sequence complementarity through a block in translation initiation not shown. PABP, poly A -binding protein AGO1-associated plant miRNAs usually share extensive sequence complementary with their mRNA targets and these interactions often result in target cleavage. However, recent studies have indicated that cleavage-independent translational repression is widespread in plants, even for highly complementary target sites. Nevertheless, miRNA-mediated cleavage is of key importance for some processes like the biogenesis of ta-siRNAs, for which the initial slicing event is key to RdRP recruitment and dsRNA synthesis. Although the purpose of this modification was initially mysterious, it is now clear that this functions as a protective group to prevent small RNA destruction ,,. In flies and mammals, small RNAs that have extensive complementarity to their targets can be recognized by terminal uridyl transferases, which mark small RNAs for degradation. The balance between protection and targeted destruction has been proposed as a quality control on small RNA sorting and as an evolutionary mechanism to drive animal miRNAs toward a cleavage-independent repression mode. They must be sorted into a particular Argonaute, AGO4, which they guide to target DNA loci by base pairing with nascent non-coding transcripts synthesized by RNAPV. Effector proteins, such as the chromatin-remodelling factor DRD1, the de novo methyltransferase DRM2 and other factors, are then recruited, resulting in DNA methylation at cytosine residues ,. As this regulation functions by repressing RNA synthesis, it was termed transcriptional gene silencing to distinguish it from post-transcriptional gene-silencing modes. Some piRNAs in flies and mammals must associate with particular Piwi-family proteins — that is, PIWI and MIWI2, respectively — which enable these small RNAs to enter the nucleus, where they are thought to induce transcriptional repression through changes in chromatin structure or DNA methylation, respectively -. Similarly, worm NRDE-3, an Argonaute of the WAGO clade, transports siRNAs to the nucleus and functions through co-transcriptional gene silencing. Thus, the final effects of small RNA sorting are felt in the modes of repression that become available as they join specific AGO proteins. The consequences of improper sorting may range from a loss of target regulation to inappropriate regulatory modes. Conclusions An understanding of the mechanisms by which small RNAs are selected and sorted among different potential effector complexes is crucial. In part, this knowledge guides hypotheses concerning the cellular roles of an ever-growing roster of small RNA species. However, the ability to predict the fate of small RNAs based on their sequence and structural characteristics is also essential to their effective use as experimental tools and potential therapeutics. We have begun to piece together the properties that determine small RNA fates and, in some instances, these properties can even predict with reasonable accuracy which small RNAs will efficiently join a particular effector complex. Yet, we still have a relatively poor ability to design effective small RNAs ab initio for experimental or therapeutic use. This capacity will rest on advances in both our understanding of RISC as an enzyme, including its mechanisms of target recognition, silencing and product release, and a detailed knowledge of how specific RNA strands are efficiently loaded into RISC as guides. The authors thank O. Zhou for comments on the manuscript. The authors are greatly indebted to J. Duffy for assistance with figures. D fellowship from the Boehringer Ingelheim Fonds. This work was supported by grants from the National Institutes of Health and a kind gift from K. Drosha and Dicer are examples of such ribonucleases. RNA-induced silencing complex A regulatory multi-protein complex containing an Argonaute protein bound to a single-stranded small RNA that regulates gene expression through sequence complementarity between the guide RNA and the target transcript. Guide strand During RISC loading, one strand of an siRNA is selected and stabilized. This is termed the guide strand, and it confers target specificity. Passenger strand The non-incorporated strand of the siRNA duplex that is degraded during the assembly of RISC. Stem—loop structure A region of dsRNA stem connected by an unpaired region loop in a single RNA molecule. This is a structure typical for miRNA precursors. Mirtron A miRNA that originates from a very short intron and is excised to form a pre-miRNA by the splicing machinery and occasionally subsequent trimming , therefore bypassing the Drosha processing step. Dicing Refers to the cleavage events carried out by the RNase III family nuclease Dicer. RNA-dependent RNA polymerase An RNA polymerase that uses ssRNA as a template to synthesize dsRNA. Trans-acting siRNA A plant small RNA that primarily associates with AGO2. Natural antisense transcript-derived siRNA A stress-induced small RNA produced by DCL1 and DCL2 that originates from dsRNA formed by convergent transcription. Heterochromatic siRNA A highly abundant plant small RNA that arises from transposons and repeats. RNase H A conserved family of endonucleases that cleave the RNA strand of RNA:DNA hybrid duplexes. AGO proteins contain RNase H-like domains.